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KMID : 0043320090320121789
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Archives of Pharmacal Research
2009 Volume.32 No. 12 p.1789 ~ p.1794
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Magnolol Induces Apoptosis via Activation of Both Mitochondrial and Death Receptor Pathways in A375-S2 Cells
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Jiao Guoqing
You Qingjun
Li Mingqiu
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Abstract
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Magnolol inhibited proliferation of human malignant melanoma A375-S2 cells. The drug induced oligonucleosomal fragmentation of DNA in A375-S2 cells and increased caspase-3, 8, 9 activities followed by the degradation of caspase-3 substrates, inhibitor of caspase dependent DNase (ICAD) and poly-(ADP-ribose) polymerase (PARP). Pan-caspase inhibitor (z-VADfmk), caspase-3 inhibitor (z-DEVD-fmk), capase-8 inhibitor (z-IETD-fmk), caspase-9 inhibitor (z-LEHD-fmk) and caspase-10 inhibitor (z-AEVD-fmk) inhibited magnolol-induced A375-S2 cell apoptosis. The level of anti-apoptotic mitochondrial protein Bcl-2 was up-regulated while the level of pro-apoptotic protein Bax was down-regulated. Taken together, our results indicate that magnolol induces apoptosis by activation of both mitochondrial and death receptor pathways in A375-S2 cells.
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KEYWORD
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Magnolol, Bax, Bcl-2, Caspase, Fas
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